DaiSyBio

To use the DaiSyBio profile, run a nf-core pipeline with -profile daisybio,<singularity/apptainer>.

This will automatically download and apply ‘daisybio.config’ as a nextflow config file.

The config file will set slurm as a scheduler for the compute cluster, define max resources, and specify cache locations for singularity, apptainer, and iGenomes. Pipeline-specific parameters still need to be configured manually.

Work directories will be kept at /nfs/scratch/nf-core_work/ in a directory named after the full path of the launch directory (”.” separated). Thy are automatically removed after a successful pipeline run. To keep the intermediate file, e.g. for using the -resume function, add keep_work as a profile: -profile daisybio,<singularity/apptainer>,keep_work.

Config file

See config file on GitHub

daisybio.config
params {
    config_profile_description = 'DaiSyBio cluster profile provided by nf-core/configs.'
    config_profile_contact = 'Johannes Kersting (@JohannesKersting)'
    config_profile_url = 'https://www.mls.ls.tum.de/daisybio/startseite/'
    max_memory = 1.TB
    max_cpus = 120
    max_time = 96.h
    igenomes_base = '/nfs/data/references/igenomes'
}
 
// define workDir in /nfs/scratch/nf-core_work/ named after the launch dir
def work_dir = "/nfs/scratch/nf-core_work/"
if(new File(work_dir).exists() && System.getenv("PWD")) {
        workDir = work_dir+System.getenv("PWD").tokenize('/').join('.')
}
 
process {
    executor = 'slurm'
    queue = 'shared-cpu'
    maxRetries = 2
}
 
executor {
    queueSize = 50
    submitRateLimit = '10 sec'
}
 
cleanup = true
profiles {
    // profile to keep work directory
    keep_work {
        cleanup = false
    }
 
    //profile for singularity
    singularity {
        singularity.enabled     = true
        singularity.autoMounts  = true
        conda.enabled           = false
        docker.enabled          = false
        podman.enabled          = false
        shifter.enabled         = false
        charliecloud.enabled    = false
        apptainer.enabled       = false
        process.beforeScript = 'module load singularity'
        singularity.cacheDir = '/nfs/scratch/singularity_cache'
    }
 
    // profile for apptainer
    apptainer {
        apptainer.enabled       = true
        apptainer.autoMounts    = true
        conda.enabled           = false
        docker.enabled          = false
        singularity.enabled     = false
        podman.enabled          = false
        shifter.enabled         = false
        charliecloud.enabled    = false
        process.beforeScript = 'module load apptainer'
        apptainer.cacheDir = '/nfs/scratch/apptainer_cache'
    }
 
}